Fig. 3. ZNF281 is highly expressed in CRC cell lines and successful knockdown of ZNF281 in SW480 and HCT116 cells. ZNF281 expression was examined by Western blotting (A) and qRT-PCR (B) in the SW620, SW480, LoVo, HT29, HCT116 and DLD-1 cell lines. (C) Western blotting was used to detect ZNF281 knockdown efficiency in SW480 and HCT116 cells. (D) qRT-PCR was used to detect ZNF281 knockdown efficiency in SW480 and HCT116 cells. Mean ± SEM, t-test, **P<0.01, ***P<0.001.